This experiment is structured to understand fundamental biogeochemical feedbacks from permafrost thaw, and address the needs of the Integrated Ecosystem Model (IEM) project whose goal is to develop a modeling framework that integrates vegetation succession, disturbance, hydrology, and permafrost dynamics using ALFRESCO, TEM, and GIPL models. Model output will also be tested or verified through field investigations in future years. The proposed research is leveraged with existing infrastructure for studying ecosystem biogeochemistry from the Yukon River Basin Project (YRB), the Alaska Peatland Experiment (APEX), and its partners.